Learn More
STEMCELL Technologies Puromycin (Dihydrochloride), Size: 50 mg

Supplier: STEMCELL Technologies 73342

Puromycin is an aminonucleoside antibiotic, derived from Streptomyces alboniger, which acts as a protein synthesis inhibitor. It binds to the target ribosome site A, where it is transferred to the growing polypeptide chain causing premature chain termination (Lührmann et al.; Rodriguez-Fonseca et al.; Azzam & Algranati). A biologically inactive form is generated when puromycin is N-acetylated by puromycin-N-acetyltransferase, allowing this gene to be used as a selective resistance marker (Vara et al. 1985).CELL LINE DEVELOPMENT· Selects for cells expressing puromycin-N-acetyltransferase resistance gene as a research tool (de la Luna & Ortín; Iwaki et al.).· Useful in CRISPR/Cas9 mammalian gene editing by selecting for successful Cas9-induced knock-in with puromycin resistance gene (Park et al.). CANCER RESEARCH· Possesses anti-tumor activity when tested against numerous cell lines (Foley & Eagle).
Safety and Handling
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.