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Sigma Aldrich Fine Chemicals Biosciences Pwo DNA Polymerase
Supplier: Sigma Aldrich Fine Chemicals Biosciences 11644955001
Pwo DNA Polymerase is a highly processive thermostable 5AE-3AE DNA polymerase and possesses a 3AE-5AE exonuclease activity also known as proofreading activity. The enzyme has no detectable 5AE-3AE exonuclease activity. Pwo DNA Polymerase exhibits increased thermal stability with a half-life of more than two hours at 100 C compared to Taq DNA Polymerase half-life of less than 5 minutes at this temperature. Pwo DNA Polymerase-generated PCR products are blunt-ended and can therefore be used directly for blunt-end ligation without any pretreatment of the ends.
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