Aviva Systems Biology Corp Pyridinoline ELISA Kit (OKEH02582) | 96 Wells

Supplier:  Aviva Systems Biology Corp OKEH02582

Aviva Systems Biology Pyridinoline ELISA Kit (OKEH02582) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Pyridinoline antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Pyridinoline and incubated. The Pyridinoline found in the sample or standards competes with the biotinylated Pyridinoline for limited binding sites on the immobilized anti-Pyridinoline antibody. Excess unbound biotinylated Pyridinoline and sample or standard Pyridinoline is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution.

Catalog No. NC1860411