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New England Biolabs, Inc. Q5 Quick-Load™ High-Fidelity 2X Master Mix - 500 reactions
Supplier: New England Biolabs, Inc. M0578L
The Q5 Quick-Load High-Fidelity 2X Master Mix offers high-fidelity performance in a convenient master mix format. The master mix features Q5, a high-fidelity, thermostable DNA polymerase with 3' to 5' exonuclease activity fused to a processivity-enhancing Sso7d domain. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 offers highly accurate amplification and can be used to amplify difficult or long targets (up to 20 kb for simple templates). The master mix includes polymerase, dNTPs, Mg++, and an inert green tracking dye for easy and direct loading of PCR products onto gels. The broad-use buffer requires only the addition of primers and DNA template for robust amplification, regardless of GC content.
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