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New England Biolabs, Inc. Q5 Site-Directed Mutagenesis Kit – 10 reactions
Supplier: New England Biolabs, Inc. E0554S
The Q5 Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid (5 minutes), room temperature circularization and template removal. Transformation into high-efficiency chemically-competent E. coli, ensures robust results with plasmids up to at least 20 kb in length.
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