Invitrogen™ Quant-iT™ 1X dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)

Catalog No.	Quantity	Assay	Quantitation Range
Q33267	1 kit	1 x dsDNA, broad range	4 to 4000 ng
Q33232	1000 assays	1 x dsDNA, high sensitivity	0.2 to 100 ng

Catalog No. Q33267 Supplier Invitrogen™ Supplier No. Q33267

Description

For easy and accurate dsDNA quantitation, the Quant-iT 1X dsDNA HS and BR Assays enable up to 1,000 fluorescence-based assays in a 96-well microplate format.

Make DNA quantitation easy and accurate in a ready-to-go 1X formulation with the Quant-iT 1X dsDNA HS (High-Sensitivity) and Quant-iT 1X dsDNA BR (Broad Range) Assay kits. Both kits provide a ready-to-use working solution. Simply load your sample, add the Qubit 1X dsDNA HS or BR working solution, and read the concentration using a fluorescence-based microplate reader.

The Quant-iT 1X dsDNA HS and BR Assay kits are highly selective for double-stranded DNA (dsDNA) over RNA, providing an accurate and precise method for the quantification of dsDNA samples. To perform the assay, dilute your sample (any volume from 1–20 μL is acceptable) into the 1X working solution provided, then read the concentration using a fluorescence plate reader.

The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/μL to 100 ng/μL, providing a core detection range of 0.2 ng to 100 ng of DNA in the assay tube. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/μL to 4000 ng/μL (depending on sample volume), providing a detection range of 4–4000 ng of DNA.

Common contaminants such as salts, free nucleotides, solvents, detergents, and protein are well tolerated in the dsDNA assays. The '1X' dsDNA assay kits provide reagent and buffer in a ready-to-use formulation and are highly selective for dsDNA over ssDNA, RNA, and protein.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

• Assay: 1 x dsDNA, broad range
• Detection Method: Fluorescence
• Excitation/Emission: 506/523
• For Use With (Equipment): Microplate Reader
• No. of Reactions: 1000 (200 μL Assay Volume)
• Quantitation Range: 4 to 4000 ng
• Product Line: Quant-iT
• Quantity: 1 kit
• Shipping Condition: This assay kit will ship as two components.
  • Quant-iT 1 x dsDNA BR Working Solution will be shipped (at ambient conditions.)
  • Quant-iT 1 x dsDNA BR lambda DNA standards will be shipped on wet ice.
  
  

Frequently Asked Questions (FAQs)

Are Cat. Nos. Q33267, Q33265 and Q33266 all the same with the same buffer?

Quant-iT 1X dsDNA Assay Kits, high sensitivity (HS) and broad range (BR) is Cat. No. Q33267.
Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits is Cat. No. Q33265.
Qubit 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits is Cat. No. Q33266.

Cat. No. Q33267 is a Quant-iT kit, while the other two are exactly same version of Qubit, just two different sizes. The kits are very similar, but optimized for different applications, and they also have a different number of standards.
Quant-iT 1X dsDNA Assay Kit, broad range (BR) (Cat. No. Q33267)
- 1 x dsDNA, broad range 4 to 4000 ng
- 1000 (200 μL Assay Volume)
Qubit 1X dsDNA Broad Range (BR) Assay Kit (Cat. No. Q33265) - 1X dsDNA, broad range 4 to 4000 ng
- 100 Reactions
Qubit 1X dsDNA Broad Range (BR) Assay Kit (Cat. No. Q33266) - 1X dsDNA, broad range 4 to 4000 ng
- 500 Reactions
The Qubit 1X dsDNA BR Assay Kits are specifically designed to be used with Qubit fluorometers, particularly the Qubit 4 and Qubit Flex models.
The Quant-iT kits can be used with both Qubit fluorometers and microplate readers, making them more versatile in terms of equipment compatibility.
However QuantiT kit can be used on Qubit and vice versa. More information about kits can be found in the Application Note: Comparison of Quant-iT and Qubit DNA quantitation assays for accuracy and precision.

What is the expiration date for the Quant-iT 1X dsDNA Assay Kits, high sensitivity (HS) and broad range (BR) (Cat. Nos. Q33232, Q3367)?

The expiration date for the Quant-iT 1X dsDNA Assay Kits, high sensitivity (HS) and broad range (BR) (Cat. Nos. Q33232, Q3367) is 6 months from the date of receipt.