Invitrogen™ Quant-iT™ microRNA Assay Kit

Description

Easily and accurately quantitate microRNA with the Quant-iT microRNA Assay Kit, which detects miRNA in pure samples down to levels as low as 0.5 ng following the supplied protocol, and even in the presence of mRNA. The assay is highly selective for microRNA over rRNA or large mRNA (>1000 bp).

The Quant-iT™ microRNA Assay Kit allows easy and accurate quantification of microRNA (miRNA), even in the presence of common contaminants such as salts, free nucleotides, solvents, detergents, and protein. The assay is highly selective for microRNA over rRNA or large mRNA (>1000 bp). Although the reagent is not exclusively selective for miRNA, we have been able to reproducibly quantify miRNA in pure samples at levels as low as 0.5 ng following the supplied protocol, even in the presence of mRNA.

Features of Quant-iT™ microRNA Assay Kit:

Accurately detects as little as 0.5 ng miRNA in a microplate well

Core dynamic range of 5–500 ng/mL

Accurate results for sample concentrations from 50 ng/mL to 100μg/mL

The Quant-iT™ microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. Simply dilute the reagent 1:200, load 200μL into the wells of a microplate, add 1–20μL of sample, mix, then read the fluorescence. The assay is performed at room temperature, and the signal is stable for approximately 3 hours. Because the assay tolerates 1–20μL of sample in an assay volume of 200μL, accurate results can be obtained for initial sample concentrations from 50 ng/mL–100μg/mL.

Which product to choose for microRNA Quantitation?

For 1–20 samples: use the Qubit™ microRNA Assay Kit with the Qubit™ Fluorometer

For 20–2,000 samples: use this Quant-iT™ microRNA Assay Kit with microplate reader

For >2,000 samples: use the Quant-iT™ microRNA Reagent with microplate reader

Discover the Qubit™ Fluorometer
The Qubit™ 2.0 Fluorometer is Life Technologies’ second generation fluorometer designed to work seamlessly with our highly specific and sensitive Qubit™ quantitation assays as well as the assays you create with the new MyQubit feature.

Introducing MyQubit
New firmware called 'MyQubit' allows you to create applications in minutes using parameters that can easily be uploaded to the instrument using a USB drive, without changing the existing assays. All new Qubit™ 2.0 instruments are pre-loaded with the new MyQubit firmware, and existing owners can download the firmware here: V3.10 firmware download. Any reagent or assay that is spectrally compatible with the Qubit™ hardware can be adapted for use with the Qubit™ 2.0 Fluorometer. Additionally we have developed assays that are ready to download, including glucose and cholesterol assays for research and drug discovery applications.

Specifications

• Assay: microRNA Quantitation
• Detection Method: Fluorescence
• Excitation/Emission: 498/518
• For Use With (Equipment): Fluorometer, Microplate Reader
• No. of Reactions: 1000 x 200 μL assays
• Quantitation Range: 1 to 100 ng
• Product Line: Quant-iT
• Quantity: 1 kit

For Research Use Only. Not for use in diagnostic procedures.