Invitrogen™ QuantiGene™ Sample Processing Kit, blood samples

Catalog No.	Quantity
QS0110	2 Plates
QS0111	10 Plates
QS0112	5 x 10 Plates

Catalog No. QS0110 Supplier Invitrogen™ Supplier No. QS0110

Description

This QuantiGene Sample Processing Kit for blood samples contains reagents and instructions for the preparation of cell lysates from whole blood samples for use in QuantiGene Singleplex and QuantiGene Plex assays for RNA or DNA targets.

This QuantiGene Sample Processing Kit for blood samples contains reagents and instructions for the preparation of cell lysates from whole blood samples for use in QuantiGene Singleplex and QuantiGene Plex assays for RNA or DNA targets.

• Easy to follow instructions
• Preserves the integrity of the RNA/DNA without degradation
• Choose the method that matches your whole blood or dried blood spot sample

What you get

The kit contains our proprietary lysis mixture, proteinase K, and detailed instructions for preparing whole blood from heparin, citrate, or EDTA tubes, PAXgene blood RNA tubes, Tempus™ blood RNA tubes, or from dried blood spots prepared from standard anticoagulant venous blood or finger sticks.

Specifications

• Content And Storage: • 10 mL Lysis Mixture, store at 15°C to 30°C
  • 625 μL Proteinase K (50 μg/μL), store at -20°C
  
  
• Format: Kit
• Sample Type: Blood
• Product Line: QuantiGene
• Product Type: Sample Processing Kit
• Quantity: 2 Plates
• Shipping Condition: Blue Ice

Frequently Asked Questions (FAQs)

Can samples in TRIzol solution be run in the QuantiGene Plex assay?

No, samples in TRIzol solution cannot be run in the QuantiGene Plex assay. However, RNA purified from these samples can be run in the QuantiGene Plex assay.

Can I use the QuantiGene Plex assay with bacteria?

Although the kits were not originally validated for bacteria, we have guidelines for preparing bacterial samples for QuantiGene Plex assays: http://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/preparation-bacterial-homogenates-quantigene-plex-assays-protocol.pdf

Do you offer QuantiGene Plex assay custom services where I can send my samples for you to process?

Yes, please reach out to your Technical Sales Specialist for more information or fill out this form: https://www.thermofisher.com/us/en/home/global/forms/life-science/request-quote-quantigene-assays.html

Can I detect genomic DNA using the QuantiGene Plex assay?

Under the hybridization conditions that are used for the QuantiGene Plex assay, genomic DNA remains double-stranded. Therefore, it is not possible for the probes to bind to DNA. The signal from the probe sets comes only from RNA.

Can I use other lysis buffers with QuantiGene Plex assay samples?

We only recommend the use of QuantiGene Lysis Mixture for preparing cell and blood lysates. The Lysis Mixture has been optimized to provide stability of RNA in the lysates. In addition, QuantiGene Lysis Mixture provides the appropriate buffer conditions for the overnight hybridization.