QuantiGene™ Singleplex HT Assay Kit

Description

QuantiGene™ Singleplex HT Assays are hybridization-based assays for the direct quantitation of RNA, DNA, or microRNA transcripts directly from cell lysates or tissue homogenates.

QuantiGene Singleplex HT assays are hybridization-based assays for the direct quantitation of RNA, DNA, or microRNA transcripts directly from cell lysates or tissue homogenates. They utilize branched DNA technology for signal amplification and signal is detected using a standard luminometer. The QuantiGene Singleplex Assay Kit contains all buffers, plates, and detection reagents necessary to perform the assay in 384-well plates.

Additional plate seals are available for separate purchase (Cat. No. QS0506).

Specifications

• Format: 384-well
• For Use With (Application): QuantiGene Singleplex Probe Set
• For Use With (Equipment): Luminometer
• Content And Storage:

  • Blocking Reagent (2 x 10 mL, store at 2°C to 8°C)
  • Pre-Amplifier Solution (3 x 400 mL, store at 2°C to 8°C)
  • Amplifier Solution (3 x 400 mL, store at 2°C to 8°C)
  • Capture Plate (50 ea, packaged individually, store at 2°C to 8°C)
  • Label Probe Solution (3 x 400 mL, store at 2°C to 8°C)
  • Substrate (4 x 300 mL, store at 2°C to 8°C)
  • Lysis Mixture (510 mL, store at 15–30°C)
  • Wash Buffer Component 1 (2 x 75 mL, store at 15–30°C)
  • Wash Buffer Component 2 (2 x 125 mL, store at 15–30°C)
  • Plate Seals (260 ea, store at 15–30°C)

• Product Line: QuantiGene Singleplex
• Product Type: Singleplex HT Assay Kit
• Quantity: 50 x 384-well plates
• Shipping Condition: Blue Ice
• Sample Type: Purified RNA, cell/blood/FFPE lysates, tissue homogenates
• Species: Universal

For Research Use Only. Not for use in diagnostic procedures.