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New England Biolabs, Inc. Quick Blunting Kit – 100 reactions
Supplier: New England Biolabs, Inc. E1201L
The Quick Blunting Kit is used to convert DNA with incompatible 5' or 3' overhangs to 5' phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA Polymerase which has both 3' to 5' exonuclease activity and 5' to 3 polymerase activity. T4 Polynucleotide Kinase is included in the enzyme mix for phosphorylation of the 5' ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 µg of DNA in a single reaction.
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