INTACT GENOMICS INC Quick10™ Cloning Kit 6 Reactions

Supplier:  INTACT GENOMICS INC 4122

Intact Genomics (IG) Quick10™ Cloning kit and DirectPlate technologies provide fast, efficient, and accurate cloning. The kit uses homologous assembly for nearly 100% accuracy, enabling direct cloning of one or two DNA fragments (<6 kb) into linearized IG AmpR/KanR-pCR vector (4 kb, included). PCR fragments are generated with IG High Fidelity i7 or other high-fidelity DNA polymerases, requiring primers with 12–15 bases of vector homology. Unpurified PCR products are diluted 5–10× in buffer and directly assembled with vector in 7 minutes at 37 °C, followed by a 3-minute transformation on ice using IG DirectPlate XL DH10B competent cells (provided). DirectPlate eliminates heat shock, long incubations, and outgrowth steps. DNA/PCR products can be cloned and transformed in ~10 minutes, versus hours for traditional methods. This system combines high-fidelity PCR, seamless assembly, and DirectPlate transformation. Custom vectors available.

Catalog No. 50-000-82821