R&D Systems™ Recombinant Human Airway Trypsin-like Protease/HAT, CF

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to cleave the fluorogenic peptide substrate, t-butoxycarbonyl-Phe-Ser-Arg-7-amino-4-methyl coumarin (Boc-FSR-AMC). The specific activity is >50,000 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: O60235
• For Use With (Application): Enzyme Activity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
• Gene ID (Entrez): 9407
• Molecular Weight (g/mol): M.W. (Observed): 27 kDa and 18 kDa, reducing conditions; M.W. (theoretical): 25 kDa & 17 kDa
• Name: Airway Trypsin-like Protease/HAT
• Quantity: 10 μg
• Source: Mouse myeloma cell line, NS0-derived human Airway Trypsin-like Protease/HAT protein Ala42-Ile418 with an N-terminal 6-His tag The protein was purified, activated and further purified.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Active
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: TMPRSS11D
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile 25 mM Tris, 150 mM NaCl and 0.05% Brij-35, pH 7.5.
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Protein: Airway Trypsin-like Protease/HAT/TMPRSS11D