R&D Systems™ Recombinant Human B7-H3 His-tag Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using mouse CD4+ cells in the presence of anti-CD3. The ED50 for this effect is 3-12 μg/mL.

Specifications

• Accession Number: NP_079516
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 80381
• Molecular Weight (g/mol): M.W. (Observed): 38-48 kDa, reducing conditions; M.W. (theoretical): 24.8 kDa
• Name: B7-H3
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human B7-H3 protein Leu29-Pro245, with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: CD276
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing (at least 0.1% human or bovine serum albumin.)
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: B7-H3/CD276