R&D Systems™ Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein, CF

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Supplier:  R&D Systems™ 356HV100/CF

Catalog No. 356HV100CF

Description

Measured by its ability to inhibit TNF-beta-mediated cytotoxicity using L-929 mouse fibroblast cells. The ED50 for this effect is 2.5-10 μg/mL in the presence of 50 pg/mL of Recombinant Human TNF-beta/TNFSF1 (Catalog # 211-TB ).

Specifications

• Accession Number: Q92956.3
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Molecular Weight (g/mol): M.W. (Observed): 60 kDa, reducing conditions; M.W. (theoretical): 45 kDa (monomer)
• Quantity: 100 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in sterile PBS.
• Protein: HVEM/TNFRSF14

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 8764
• Name: HVEM/TNFRSF14 Fc Chimera
• Source: Mouse myeloma cell line, NS0-derived human HVEM/TNFRSF14 protein Human HVEM Pro37-Val202 (Ser108Thr; Ala140Arg) (N-terminus) Accession # Q92956.3 IEGRMD Human IgG1 (Pro100-Lys330) 6-His tag (C-terminus)
• Structural Form: Disulfide-linked homodimer
• Gene Symbol: TNFRSF14
• Recombinant: Recombinant
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.