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R&D Systems™ Recombinant Human LIGHT/TNFSF14 Protein

Catalog No. 664LI025
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Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
This item is not returnable. View return policy
This item is not returnable. View return policy

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 0.3-3 ng/mL.

Specifications

Accession Number O43557
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Gene ID (Entrez) 8740
Molecular Weight (g/mol) M.W. (Observed): 25 kDa, reducing conditions; M.W. (theoretical): 20.9 kDa
Name LIGHT/TNFSF14
Quantity 25 μg
Source Mouse myeloma cell line, NS0-derived human LIGHT/TNFSF14 protein Met 10-His tag GGGSGGGSGGGSIEGR Human Light (Asp74-Val240) (N-terminus) Accession # O43557 (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Symbol TNFSF14
Conjugate Unconjugated
Recombinant Recombinant
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing (at least 0.1% human or bovine serum albumin.)
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein LIGHT/TNFSF14
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