R&D Systems™ Recombinant Human LIGHT/TNFSF14 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 0.3-3 ng/mL.

Specifications

• Accession Number: O43557
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 8740
• Molecular Weight (g/mol): M.W. (Observed): 25 kDa, reducing conditions; M.W. (theoretical): 20.9 kDa
• Name: LIGHT/TNFSF14
• Quantity: 25 μg
• Source: Mouse myeloma cell line, NS0-derived human LIGHT/TNFSF14 protein Met 10-His tag GGGSGGGSGGGSIEGR Human Light (Asp74-Val240) (N-terminus) Accession # O43557 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: TNFSF14
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing (at least 0.1% human or bovine serum albumin.)
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: LIGHT/TNFSF14