R&D Systems™ Recombinant Human TGF-beta 1 Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Supplier:  R&D Systems™ 240B010

Catalog No. 240B010

Description

Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.04-0.2 ng/mL.

Specifications

• Accession Number: P01137
• Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Molecular Weight (g/mol): M.W. (Observed): 12 kDa, reducing conditions 24 kDa, non-reducing conditions; M.W. (theoretical): 12.8 kDa (monomer)
• Quantity: 10 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: TGFB1
• Recombinant: Recombinant
• Purity or Quality Grade: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 7040
• Name: TGF-beta 1
• Source: Chinese Hamster Ovary cell line, CHO-derived human TGF-beta 1 protein Ala279-Ser390
• Structural Form: Disulfide-linked homodimer
• Gene Alias: CEDLAP, DPD1, latency-associated peptide, TGF beta1, TGFB, TGFB1, TGFbeta, TGFbeta 1, TGF-beta 1 protein, TGF-beta-1, transforming growth factor beta-1, transforming growth factor, beta 1
• Conjugate: Unconjugated
• Reconstitution: Purified recombinant human TGF-beta 1 is an extremely hydrophobic protein that adheres strongly to surfaces. To ensure recovery, reconstitute 2 μg vials at 20 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin. Reconstitute 10 μg or larger vials at 100 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin.
• Protein: TGF-beta 1