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R&D Systems™ Recombinant Human VEGF 165b Protein

Catalog No. 3045VE025
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Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Lyophilized from a 0.2μm filtered solution in PBS.
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Catalog No. Formulation
3045VE025 Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
3045VE025CF Lyophilized from a 0.2μm filtered solution in PBS.
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its binding ability in a functional ELISA. Serially diluted Recombinant Human (rh) VEGF165b is added to a Recombinant Human VEGF R2/KDR/Flk-1 Fc Chimera (Catalog # 357-KD ) coated plate (2 μg/mL, 100 μL/well). Bound rhVEGF165b is detected by a human VEGF165b specific antibody (Catalog # MAB3045 ). The concentration of rhVEGF165b that produces half-maximum response is approximately 0.12-0.6 nM.

Specifications

Accession Number AAL27435
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Gene ID (Entrez) 7422
Molecular Weight (g/mol) M.W. (Observed): 19-24 kDa, reducing conditions; M.W. (theoretical): 19.1 kDa (monomer)
Name VEGF 165b
Quantity 25 μg
Source Spodoptera frugiperda, Sf 21 (stably transfected)-derived human VEGF protein Ala27-Asp191
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Structural Form Disulfide-linked homodimer
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Symbol VEGFA
Conjugate Unconjugated
Recombinant Recombinant
Reconstitution Reconstitute at 100 μg/mL in PBS containing (at least 0.1% human or bovine serum albumin.)
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein VEGF
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