R&D Systems™ Recombinant Mouse SIRP alpha/CD172a Fc Chimera Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by the ability of the immobilized protein to support the adhesion of mouse red blood cells.

The ED50 for this effect is 0.4-1.6 μg/mL.

Optimal dilutions should be determined by each laboratory for each application.

Specifications

• Accession Number: P97797
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 140885
• Molecular Weight (g/mol): M.W. (Observed): 115-125 kDa, reducing conditions; M.W. (theoretical): 65.1 kDa (monomer)
• Name: SIRP alpha/CD172a Fc Chimera
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived mouse SIRP alpha/CD172a protein Mouse SIRP alpha/CD172a (Met1-Asn373) (Gly365Asp) (N-terminus) Accession # P97797 IEGRMDP Mouse IgG2A (Glu98-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: Sirpa
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: SIRP alpha/CD172a