R&D Systems™ Recombinant Mouse SIRP alpha/CD172a Fc Chimera Protein, CF

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Manufacturer:  R&D Systems™ 7154SA050

Catalog No. 7154SA050

Description

Measured by the ability of the immobilized protein to support the adhesion of mouse red blood cells.

The ED50 for this effect is 0.4-1.6 μg/mL.

Optimal dilutions should be determined by each laboratory for each application.

Specifications

• Accession Number: P97797
• Formulation: Lyophilized from a 0.2 µm filtered solution in PBS.
• Molecular Weight: M.W. (Observed): 115-125 kDa, reducing conditions; M.W. (theoretical): 65.1 kDa (monomer)
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Source: Mouse myeloma cell line, NS0-derived mouse SIRP alpha/CD172a protein Mouse SIRP alpha/CD172a (Met1-Asn373) (Gly365Asp) (N-terminus) Accession # P97797 IEGRMDP Mouse IgG2A (Glu98-Lys330) (C-terminus)
• Structural Form: Disulfide-linked homodimer
• Gene Symbol: Sirpa
• Recombinant: Yes
• Protein: SIRP alpha/CD172a

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 140885
• Name: SIRP alpha/CD172a Fc Chimera
• Quantity: 50 µg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <1.0 EU / 1 µg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 µg/mL in PBS.