R&D Systems™ Recombinant Mouse VISTA/B7-H5/PD-1H Fc Chimera Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-3 μg/mL.

Order Info

The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended storage temperatures.

Specifications

• Accession Number: Q9D659
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 64115
• Molecular Weight (g/mol): M.W. (Observed): 60-70 kDa, reducing conditions; M.W. (theoretical): 45 kDa (monomer)
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived mouse VISTA/B7-H5/PD-1 H protein Mouse VISTA/B7-H5/PD-1 H (Phe33-Ala191) (N-terminus) Accession # NP_001153044 IEGRMDP Mouse IgG2A (Glu98-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: Vsir
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE with silver staining.
• Protein: VISTA/B7-H5/PD-1 H