Invitrogen™ Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor™ Plus 647

Catalog No.	Quantity
50-003-31946	250 μL

Catalog No. 50-003-31946 Supplier Invitrogen™ Supplier No. SA510386AFP647

Description

Rabbit Recombinant Superclonal Secondary Antibody

The Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ Plus 647 specifically detects the heavy and the light chains of goat IgG. It has ~20% cross reactivity to Hamster IgG and showed minimum to no cross-reactivity to various species immunoglobulins tested. This recombinant antibody has been engineered with point mutations that eliminate binding to Fc receptors, enabling reduction in background and superior signal to noise. The sensitivity and specificity of each lot is confirmed using ELISA. Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency. Alexa Fluor™ Plus dyes have been chemically optimized to provide higher signal to noise ratios and minimized cross-reactivity to help detect low-abundance targets in rare or precious samples. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Goat IgG (H+L)
• Applications: Immunohistochemistry (Paraffin), Immunocytochemistry
• Classification: Recombinant Superclonal
• Concentration: 1 mg/mL
• Conjugate: Alexa Fluor Plus 647
• Formulation: Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.3-7.3
• Host Species: Rabbit
• Immunogen: Purified Goat IgG.
• Purification Method: Affinity chromatography
• Quantity: 250 μL
• Regulatory Status: RUO
• Primary or Secondary: Secondary
• Target Species: Goat
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Secondary Antibody
• Form: Liquid
• Isotype: IgG