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Invitrogen™ Rabbit anti-Human IgG4 Recombinant Secondary Antibody

Catalog No. PISA510205
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100 μg
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PISA510205 100 μg
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Catalog No. PISA510205 Supplier Invitrogen™ Supplier No. SA510205
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Rabbit Recombinant Monoclonal Secondary Antibody

This antibody reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Human IgG4
Applications ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blot
Classification Recombinant Monoclonal
Clone RM120
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 1% BSA, 50% glycerol and 0.09% sodium azide; pH 7.2-7.4
Host Species Rabbit
Immunogen Peptide corresponding to the hinge region of Human IgG4.
Purification Method Protein A
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Human
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Product Type Secondary Antibody
Form Liquid
Isotype IgG
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