Invitrogen™ Rabbit anti-Mouse IgG Fc Superclonal™ Secondary Antibody, PE

Catalog No.	Quantity
50-005-55620	500 μL

Catalog No. 50-005-55620 Supplier Invitrogen™ Supplier No. SA510390PE

Description

Rabbit Recombinant Superclonal Secondary Antibody

Rabbit anti-Mouse IgG Fc Superclonal™ Recombinant Secondary Antibody, PE is specific to Fc fragment of Mouse IgG. It can detect all subtypes of Mouse IgG (Mouse IgG1, 2a, 2b and 3). It has less than 15% cross reactivity to Human IgG. Minimal to no cross-reactivity was observed with immunoglobulins from the other species tested. This recombinant antibody has been engineered with point mutations that eliminate binding to Fc receptors, enabling reduction in background and superior signal to noise. Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency. R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity, but not photostability. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Mouse IgG Fc
• Applications: Flow Cytometry
• Classification: Recombinant Superclonal
• Concentration: 0.2 mg/mL
• Conjugate: PE
• Formulation: PBS with 5mM sodium azide; pH 7.4
• Host Species: Rabbit
• Immunogen: Purified Rabbit IgG
• Purification Method: Affinity chromatography
• Quantity: 500 μL
• Regulatory Status: RUO
• Primary or Secondary: Secondary
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Secondary Antibody
• Form: Liquid
• Isotype: IgG