Invitrogen™ Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, TRITC

Rabbit Polyclonal Secondary Antibody

Supplier:  Invitrogen™ A16171

Catalog No. A16171

Description

Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid. Based on Immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins, human serum proteins.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Mouse IgG (H+L) Cross-Adsorbed
• Classification: Polyclonal
• Conjugate: TRITC
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Mouse
• Product Type: Secondary Antibody
• Isotype: IgG

• Applications: Immunocytochemistry
• Concentration: 2.5 mg/mL
• Formulation: PBS with 1% BSA and 0.05% sodium azide; pH 7.2
• Immunogen: Gamma Immunoglobins Heavy and Light chains.
• Quantity: 1 mg
• Primary or Secondary: Secondary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
• Form: Lyophilized

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov