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Sigma Aldrich Fine Chemicals Biosciences rAPid Alkaline Phosphatase
Supplier: Sigma Aldrich Fine Chemicals Biosciences 4898133001
rAPid Alkaline Phosphatase is an alkaline phosphatase isolated from bovine intestine and supplied as a recombinant enzyme of 56 kD expressed in the yeast Pichia Pastoris. The recombinant form ensures consistency and safety. rAPid Alkaline Phosphatase catalyzes the dephosphorylation of 5 phosphates from DNA and RNA nucleotides and proteins. Alkaline phosphatase treatment prevents self-ligation of DNA fragments by removing the 5-phosphoryl termini required by ligases this feature is of major importance in cloning strategies to decrease vector background. Unlike calf intestinal phosphatase rAPid Alkaline Phosphatase is rapidly completely and irreversibly inactivated by heat treatment for two minutes at 75 C. It is therefore an excellent alternative to Shrimp Alkaline Phosphatase. In addition the enzyme is active in restriction enzyme buffers. Therefore restriction enzyme digestion dephosphorylation enzyme inactivation ligation or 5-end labeling can be performed without purif
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