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MP Biomedicals™ Rapid-DNA Blood/Cell/Tissue Kit
Description
The Rapid-DNA Blood/Cell/Tissue Kit is a fast and easy method to obtain high quality genomic DNA from whole blood, cultured cells, buffy coats, tissues and other body fluids. The easy to use, rapid DNA extraction protocols can process multiple types of samples in a single kit and is ideal for the extraction of pure, ready-to-use, high molecular weight genomic DNA in a minimal amount of time. Genomic DNA extracted with this kit can be used with all downstream applications such as PCR, qPCR, Next Generation sequencing, restriction digests and cloning.
MP Biomedical provides the fully equipped Rapid-DNA extraction kit to isolate genomic DNA from any sample type of blood, cells, and tissues at high quality for research analysis and downstream applications.
- High-Quality DNA Purification
- Pure genomic DNA Isolation
- All-in-One Kit for Blood, Cells, and Tissue
- Ideal for all Downstream Applications (ie. PCR, Restriction Digestion, Sequencing)
In this Rapid-DNA extraction kit, lysing matrix is provided to process a range of tissues and/or organs. Samples are placed into 2.0 mL tubes containing Lysing Matrix M, a 1/4-inch ceramic beads designed to efficiently lyse a variety of tissue samples. Homogenization in a FastPrep instrument with Lysing Matrix M takes place in the presence of lysing buffer that is provided within this kit and validated to protect and solubilize nucleic acids and proteins upon cell lysis.
Depending on the expected yield of genomic DNA, the DNA can also be eluted with a lower or a higher volume of Elution Buffer. The minimum elution volume is 50 μL. If quite large amount of DNA is expected, the volume of elution can be increased (100 to 200 μL). To maximize the final yield, we recommend a second elution step with an equal volume of Elution Buffer.
Specifications
Specifications
| Column Type | Silica Spin Filter |
| Format | Kit |
| Sample Type | Whole Blood, Cultured Cells, Buffy Coats, Tissues, Body Fluids, Cell Cultures, Buffy Coat, Tissue, Body Fluids |
| Elution Volume | 50 to 200 μL |
| For Use With (Application) | Blood, cells and tissues to isolate genomic DNA |
| Matrix | Without Matrix |
| No. of Reactions | 50 |
| Nucleic Acid Type | Genomic DNA |
| Product Type | DNA Isolation Kit |
| Shipping Condition | Ambient |
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use.
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