Invitrogen™ Rat Angiopoietin-1 ELISA Kit

Description

ELISA

Rat Angiopoietin-1 quantitates rat Angiopoietin-1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant rat Angiopoietin-1.

Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

Specifications

• Accession Number: 1110046O21Rik; AGP1; AGPT; Agpt1; Ang1; Ang-1; angiopoietin 1; angiopoietin-1; ANGPT1; endothelial growth factor; KIAA0003
• Assay Range: 0.4 to 100 ng/mL
• Assay Sensitivity: 0.4 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 89807
• Gene Symbol: Angpt1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Angiopoietin 1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins