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Invitrogen™ Rat anti-Mouse IgG1 Secondary Antibody, HRP, eBioscience™
Description
Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies. Applications Reported: This M1-14D12 antibody has been reported for use in western blotting, ELISA, immunohistochemical and immunocytochemical staining. Applications Tested: This M1-14D12 antibody has been tested by immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 20 μg/mL. For western blotting, this can be used at 1 μg/mL (1/500 dilution). It is recommended that the antibody be titrated for optimal performance in the assay of interest.
Specifications
Specifications
| Antigen | Mouse IgG1 |
| Applications | ELISA, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry |
| Classification | Monoclonal |
| Clone | M1-14D12 |
| Concentration | 0.5 mg/mL |
| Conjugate | HRP |
| Formulation | PBS with 50% glycerol and no preservative; pH 7.2 |
| Host Species | Rat |
| Purification Method | Affinity chromatography |
| Quantity | 100 μg |
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