Invitrogen™ Rat anti-Mouse IgG1 Secondary Antibody, HRP, eBioscience™

Rat Monoclonal Secondary Antibody

Supplier:  Invitrogen™ 18401582

Catalog No. 50-112-8837

Description

Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies. Applications Reported: This M1-14D12 antibody has been reported for use in western blotting, ELISA, immunohistochemical and immunocytochemical staining. Applications Tested: This M1-14D12 antibody has been tested by immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 20 μg/mL. For western blotting, this can be used at 1 μg/mL (1/500 dilution). It is recommended that the antibody be titrated for optimal performance in the assay of interest.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Mouse IgG1
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 50% glycerol and no preservative; pH 7.2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Mouse
• Product Type: Secondary Antibody
• Isotype: IgG

• Applications: Immunohistochemistry (Paraffin), Western Blot, ELISA, Immunocytochemistry
• Clone: M1-14D12
• Conjugate: HRP
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Secondary
• Content And Storage: 4°C
• Form: Liquid