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BIOHIPPO Rat C5a Microsample

Supplier: BIOHIPPO BHE15212224
Rat C5a Microsample ELISA Kit is a Sandwich-based immunoassay designed for the quantitative measurement of C5a in Serum plasma and other biological fluids It provides a detection range of 1 57-100 ng/mL with a sensitivity of 0 63 ng/mL The assay can be completed in approximately 3 5h The procedure follows a The test principle applied in this kit is Sandwich enzyme immunoassay The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat C5a Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat C5a Next Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated After TMB substrate solution is added only those wells that contain Rat C5a biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color
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