Invitrogen™ Rat Complement C4 ELISA Kit

Catalog No.	Quantity
EEL225	96 Tests

Catalog No. EEL225 Supplier Invitrogen™ Supplier No. EEL225

Description

ELISA

Rat Complement C4 ELISA Kit quantifies Complement C4 in serum and plasma. The Rat Complement C4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene.

Specifications

• Accession Number: P08649
• Assay Range: 3.13-200 ng/mL
• Assay Sensitivity: 1.88 ng/mL
• Conjugate: HRP
• Detection Method: Colorimetric
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Rat
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: acidic C4; acidic complement C4; Basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3; C4; c4 complement; C4-1; C4a; C4a anaphylatoxin; C4A2; C4A3; C4A4; C4A6; C4AD; C4B; C4B_2; C4B1; C4B12; C4B2; C4B3; C4B5; C4b-A; C4b-B; C4BD; C4d-A; C4d-B; C4F; C4S; CH; Chido form of C4; CO4; Complement C4; Complement C4 alpha chain; Complement C4 beta chain; Complement C4 gamma chain; Complement C4-A; Complement C4-A alpha chain; Complement C4-B; Complement C4-B alpha chain; complement C4B1a; complement component 4 (within H-2S); complement component 4, gene 1; complement component 4A (Rodgers blood group); complement component 4B (Chido blood group); complement component 4B (Childo blood group); CPAMD2; CPAMD3; DADB-112B14.11; DAMC-258G8.6; FLJ60561; MGC164979; MHC class III region complement; RG; Rodgers form of C4; sex-limited protein; Slp; Ssacidic C4; acidic complement C4; Basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3; C4; c4 complement; C4-1; C4a; C4a anaphylatoxin; C4A2; C4A3; C4A4; C4A6; C4AD; C4B; C4B_2; C4B1; C4B12; C4B2; C4B3; C4B5; C4b-A; C4b-B; C4BD; C4d-A; C4d-B; C4F; C4S; CH; Chido form of C4; CO4; Complement C4; Complement C4 alpha chain; Complement C4 beta chain; Complement C4 gamma chain; Complement C4-A; Complement C4-A alpha chain; Complement C4-B; Complement C4-B alpha chain; complement C4B1a; complement component 4 (within H-2S); complement component 4, gene 1; complement component 4A (Rodgers blood group); complement component 4B (Chido blood group); complement component 4B (Childo blood group); CPAMD2; CPAMD3; DADB-112B14.11; DAMC-258G8.6; FLJ60561; MGC164979; MHC class III region complement; RG; Rodgers form of C4; sex-limited protein; Slp; Ss;
• Gene ID (Entrez): 24233, 406161
• Gene Symbol: C4A, C4B
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Micro ELISA Plate (Dismountable), Reference Standard , Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis, Reference Standard, Concentrated Biotinylated Detection Ab(100x), Concentrated HRP Conjugate (100x), Reference Standard & Sample Diluent, Biotinylated Detection Ab Diluent, HRP Conjugate Diluent, Concentrated Wash Buffer(25x), Substrate Reagent, Stop Solution, Plate Sealer, Product Description, Certificate of Analysis
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma: 100 μL, Serum: 100 μL
• Shipping Condition: Wet Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Complement C4
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min