Invitrogen™ Rat Corticosterone Rapid ELISA Kit

Catalog No.	Quantity
EELR016	96 Tests

Catalog No. EELR016 Supplier Invitrogen™ Supplier No. EELR016

Description

ELISA

The Rat Corticosterone (CORT) ELISA quantitates CORT in serum, plasma, urine, saliva. Principle of the method The Rat Cort solid phase competitive ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between the pre coated antigen and the HRP linked antibody specific for Rat Cort. A target specific antigen has been pre coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells, where Rat Cort in the samples (or standards) competes with the immobilized (capture) antigen for sites on the HRP linked detection antibody. Excess conjugate and unbound sample or standard are washed away from the plate. The substrate solution is added to each well, and the enzyme substrate reaction is terminated by the addition of a stop solution, turning the color yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The intensity of this signal is inversely proportional to the concentration of Rat Cort present in the original specimen, which is determined by comparing the OD of the samples to the standard curve. Achieve fast and reliable results in just 90 minutes with our Rapid ELISA kit, featuring a streamlined protocol that requires only one wash step. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more informati...

CORTICOSTERONE is an adrenocortical steroid that has modest but significant activities as a mineralocorticoid and a glucocorticoid. [National Center for Biotechnology Information. PubChem Compound Database; CID=5753].

Specifications

• Assay Range: 4.69-300 ng/mL
• Assay Sensitivity: 1.87 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Saliva, Serum, Urine
• Target Species: Rat
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 17-Deoxycortisol; C21H30O417-Deoxycortisol; C21H30O4;
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Saliva 50 μL, Serum 50 μL, Urine 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Corticosterone
• Test Time: 15 min
• Total Assay Time: 1 hr 30 min