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Innovative Research Inc Rat Epiregulin Kit
Supplier: Innovative Research Inc IRTEREGKT
Rat Epiregulin ELISA Kit - Rat Epiregulin ELISA Kit from Innovative Research is intended for the quantitative determination of Rat Epiregulin in biofluid samples, such as serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . EREG present in samples reacts with an anti-Rat EREG capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-EREG detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active EREG in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Rat EREG.
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