Rat ET-1 Microsample ELISA Kit is a Sandwich-based immunoassay designed for the quantitative measurement of ET-1 in Serum plasma tissue homogenates cell lysates cell culture supernates and other biological fluids It provides a detection range of 7 82-500 pg/mL with a sensitivity of 2 67 pg/mL The assay can be completed in approximately 3 5h The procedure follows a The test principle applied in this kit is Sandwich enzyme immunoassay The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ET-1 Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ET-1 Next Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated After TMB substrate solution is added only those wells that contain Rat ET-1 biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color