Invitrogen™ Rat FSH ELISA Kit

Catalog No.	Quantity
EEL125	96 Tests

Catalog No. EEL125 Supplier Invitrogen™ Supplier No. EEL125

Description

ELISA

The Rat FSH (Follicle Stimulating Hormone) ELISA quantitates FSH in serum, plasma, and other biological fluids The Rat FSH (Follicle Stimulating Hormone) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Follicle-stimulatine hormone (FSH) is a member of the glycoprotein hormone family. Members of this family consist of an identical alpha subunit and a hormone-specific beta subunit. Alternative splicing results in two transcript variants encoding the same protein. FSH is secreted by gonadotropic cells in the pituitary gland and in conjunction with luteinizing hormone, induces egg and sperm production.

Specifications

• Accession Number: P11962, P18427
• Assay Range: 3.13-200 ng/mL
• Assay Sensitivity: 1.88 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Glycoprotein hormone
• Sample Type: Plasma, Serum
• Target Species: Rat
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: class 1 follicle-stimulating hormone beta polypeptide; class 2 follicle-stimulating hormone beta polypeptide; follicle - stimulating hormone subunit beta; follicle stimulating hormone beta; follicle stimulating hormone beta subunit; follicle stimulating hormone beta-subunit precursor; follicle stimulating hormone subunit beta; follicle stimulating hormone, beta polypeptide; follicle-stimulating hormone beta subunit; follicle-stimulating hormone beta-subunit; follicle-stimulating hormone subunit beta; follicule stimulating hormone, beta polypeptide; follitropin beta chain; Follitropin subunit beta; follitropin, beta chain; FSH; FSH-alpha; FSHB; FSH-B; Fshbeta; FSH-beta; HH24; LSH-alpha; prefollicle stimulating hormone beta chain; TSH-alphaclass 1 follicle-stimulating hormone beta polypeptide; class 2 follicle-stimulating hormone beta polypeptide; follicle - stimulating hormone subunit beta; follicle stimulating hormone beta; follicle stimulating hormone beta subunit; follicle stimulating hormone beta-subunit precursor; follicle stimulating hormone subunit beta; follicle stimulating hormone, beta polypeptide; follicle-stimulating hormone beta subunit; follicle-stimulating hormone beta-subunit; follicle-stimulating hormone subunit beta; follicule stimulating hormone, beta polypeptide; follitropin beta chain; Follitropin subunit beta; follitropin, beta chain; FSH; FSH-alpha; FSHB; FSH-B; Fshbeta; FSH-beta; HH24; LSH-alpha; prefollicle stimulating hormone beta chain; TSH-alpha;
• Gene ID (Entrez): 116700, 25447
• Gene Symbol: CGA, FSHB
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Follicle Stimulating Hormone
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min