Rat HMGB-1 Microsample ELISA Kit is a Sandwich-based immunoassay designed for the quantitative measurement of HMGB-1 in serum plasma tissue homogenates and other biological fluids It provides a detection range of 0 16-10 ng/mL with a sensitivity of 0 067 ng/mL The assay can be completed in approximately 3 5h The procedure follows a The test principle applied in this kit is Sandwich enzyme immunoassay The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HMGB-1 Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HMGB-1 Next Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated After TMB substrate solution is added only those wells that contain Rat HMGB-1 biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color