Invitrogen™ Rat IFN-alpha 2 Recombinant Protein

Catalog No.	Quantity
PI131001	1 x 10^5 units

Catalog No. PI131001 Supplier Invitrogen™ Supplier No. 131001

Description

Recombinant Protein

Each blood donation used for interferon production tested negative for: HBsAg, HIV I-II, HCV and VDRL. After receipt, this product should be kept at 4°C while lyophilized and at -70°C after reconstitution in sterile distilled water for retention of full activity. After reconstitution store at -70°C in aliquots. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Further dilute only in buffers containing protein such as 0.1% bovine serum albumin (BSA).

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.

Specifications

• For Use With (Application): ELISA, Immunoprecipitation, Western Blot
• Formulation: citric acid with 0.125 M trehalose, NaCl and no preservative; pH 5
• Gene ID (Entrez): 298213
• Molecular Weight (g/mol): 19 kDa
• Name: Rat IFN-alpha 2
• pH Range: 5
• Quantity: 1 x 10^5 units
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: alpha-2 A interferon; Ifa2; IFNA; IFN-a; IFNA 2; Ifna2; IFNA2A; IFNA2B; IFNA2C; IFN-alpha 1 b; IFN-alpha-1/13; IFN-alpha-2; IFN-alphaA; INFA2; Interferon; interferon alpha 1 b; interferon alpha 2; interferon alpha 2 A; interferon alpha 2 b; interferon alpha A; interferon alpha family, gene 2; interferon alpha-2; Interferon alpha-A; interferon alpha-D; interferon, alpha 2; Interferon-alpha II; leIF A; leIF D; RP11-354P17.2
• Common Name: Interferon alpha-2
• Gene Symbol: Ifna2
• Biological Activity: EC50 ̃ 1 U/mL; determined by use of the cytopathic effect inhibition assay described in [Rubinstein, S. et al. (1981). J. Virol. 37, 755-758 ]; [Familletti, P.C. et al. (1981). Methods in Enzymology, Vol. 78, 387-394 ]As there is no international Rat IFN-alpha standard, units are determined with respect to the international reference standard for mouse interferon alpha/beta (mouse IFN-alpha/beta) provided by the National Institutes of Health.
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Content And Storage: 4°C
• Expression System: E. coli
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: >95%