Invitrogen™ IFN gamma Rat ELISA Kit

Measure levels of rat interferon gamma (IFNg) in rat serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples using 96-well plates.

Catalog Number	Mfr. No.	Quantity
ENERIFNG	Invitrogen™ ERIFNG	96 Tests
ENERIFNG2	Invitrogen™ ERIFNG2	2 x 96 Tests

Description

Assay IFN-γ(rat interferon gamma) levels in rat serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Rat IFN gamma ELISA Kits.

These rat IFN-gamma ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: Natural and recombinant rat interferon gamma (IFNγ)

• Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

• Measurement: Absorbance at 450nm minus absorbance at 550nm

• Assay Range: 8 to 500pg/mL

• Sensitivity: < 2pg/mL

• Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

• Total Assay Time: 2.5 hours

• Sample Size: 50μL

• Specificity: No significant cross-reaction with rat IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, MIP-1α, RANTES, or TNFα, or human or pig IFNγ. When preparations of NIH/NIBSC mouse IFNγ (lot Gg02-901-533) were evaluated in the Rat IFNγ ELISA Kit, 6250pg/mL mouse IFNγ was equivalent to 258pg/mL rat IFNγ (4.1% cross-reactivity)

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

• Accession Number: P01581
• Assay Sensitivity: <2pg/mL
• Conjugate: Biotin
• Product Type: ELISA Kit
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IFNG2
• Intraassay CV: 8.5%
• Measurement: Absorbance at 450nm minus absorbance at 550nm
• Sample Volume: Plasma,50μL; Serum,50μL; Supernatant,50μL
• Target: Rat
• Total Assay Time: 2 hr. 30 min.

• Assay Range: 8 to 500pg/mL
• Description: Rat IFN gamma ELISA Kit
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Sample Type: Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
• Gene ID (Entrez): 25712
• Interassay CV: 8.7%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.