Invitrogen™ Rat IgE ELISA Kit

Catalog No.	Quantity
EEL126	96 Tests

Catalog No. EEL126 Supplier Invitrogen™ Supplier No. EEL126

Description

ELISA

The Rat IgE ELISA quantitates IgE in serum, plasma, and other biological fluids The Rat IgE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Rat IgE, also referred to as rat immunoglobulin E, is an antibody isotype synthesized by rats in response to allergens or parasites. It plays a pivotal role in mediating allergic reactions and immune responses against specific pathogens. IgE antibodies are primarily responsible for eliciting the release of inflammatory mediators, such as histamine, from mast cells and basophils. This immune response can give rise to symptoms like pruritus, edema, and bronchoconstriction. Investigating the genetic target Rat IgE can provide valuable insights into the underlying mechanisms of allergic diseases and contribute to the development of innovative therapeutic strategies for allergies and immunological disorders in both rats and humans.

Specifications

• Accession Number: P01855
• Assay Range: 1.56-100 ng/mL
• Assay Sensitivity: 0.94 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Immunoglobulin
• Sample Type: Plasma, Serum
• Target Species: Rat
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Immunoglobulin E; ImmunoglobulinEImmunoglobulin E; ImmunoglobulinE;
• Gene Symbol: IGHE
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Rat IgE
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min