Gibco™ Rat IL-10 Recombinant Protein

Description

Recombinant Protein

Reconstitution: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized rat IL-10 in 5 mM Tris, pH 7.2, to a concentration of 0.1-1.0 mg/mL. Further dilution should be made in medium or buffered solution containing carrier protein, such as PBS with 0.1% BSA. Storage: This lyophilized preparation is stable at 2°C to 8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2°C to 8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C. Avoid repeated freeze/thaw cycles.

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

Specifications

• Accession Number: P29456
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: 5 mM tris with no preservative; pH 7.2
• Gene ID (Entrez): 25325
• Molecular Weight (g/mol): 18.7 kDa
• Name: Rat IL-10
• Purification Method: Affinity Chromatography
• Quantity: 10 μg
• Endotoxin Concentration: <0.1 ng/ μg
• Gene Alias: CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF
• Common Name: IL-10
• Biological Activity: ED50 ≤ 10 ng/mL; determined by the dose-dependent inhibition of antigen-specific T-cells.
• Conjugate: Unconjugated
• Species: Rat
• Recombinant: Recombinant
• Sequence: Rat IL-10 recombinant protein contains 160 amino acids
• Content And Storage: -20°C
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Interleukins
• Research Category: Immunology, Inflammation, Signal Transduction
• Product Line: Gibco™
• Purity or Quality Grade: >98% by SDS-PAGE and HPLC