Invitrogen™ Rat IL-2 ELISA Kit

Description

ELISA

Rat IL-2 quantitates rat IL-2 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant rat IL-2.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

Specifications

• Accession Number: Aldesleukin; cytokine; H-IL-2; il 2; Il2; IL-2; ILN; Interleukin; interleukin 2; interleukin 2 precursor; Interleukin2; interleukin-2; interleukin-2 precursor; interleukin-2 precursor protein; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; POIL2; porcine Interleukin 2; T cell growth factor; T-cell growth factor; TCGF
• Assay Range: 0.1 to 30 ng/mL
• Assay Sensitivity: 0.1 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 116562
• Gene Symbol: IL2
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: IL-2
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov