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Invitrogen™ Rat JAM-A ELISA Kit

Catalog No. ERA36RBX5
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ELISA

Rat JAM-A quantitates rat JAM-A in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant rat JAM-A.

CD321 (F11R, JAM-1, JAM-A) is a cell-surface adhesion molecule belonging to the Ig superfamily of receptors. CD321 is a 32-41 kD glycoprotein, possessing two extracellular Ig-like domains, a transmembrane region, and a short cytosolic tail. The protein assembles at the membrane as a homodimer, and can associate laterally with other membrane proteins including integrins. CD321 is expressed on a variety of cell types, including leukocytes, platelets, erythrocytes, stem cells, epithelial and endothelial cells, and plays a role in mediating cell-cell contact. CD321 can be found in close proximity to several components of epithelial tight junctions, including ZO-1 and occludin. CD321 is involved in the maintenance of epithelial and endothelial barriers, hematopoiesis, angiogenesis, and tumor proliferation, invasion, and metastasis.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q9JHY1
Assay Range 7-1500 pg/mL
Assay Sensitivity 7 pg/mL
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Rat
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias 9130004G24; AA638916; BV11 antigen; CD321; ESTM33; F11 R; F11 receptor; F11 receptor/F11R; F11R; JAM; JAM1; JAM-1; JAMA; JAM-A; Jcam; Jcam1; junction cell adhesion molecule A; junction cell adhesion molecule1; junctional adhesion molecule 1; Junctional adhesion molecule A; KAT; Ly106; PAM-1; Platelet adhesion molecule 1; platelet F11 receptor; UNQ264/PRO3019130004G24; AA638916; BV11 antigen; CD321; ESTM33; F11 R; F11 receptor; F11 receptor/F11R; F11R; JAM; JAM1; JAM-1; JAMA; JAM-A; Jcam; Jcam1; junction cell adhesion molecule A; junction cell adhesion molecule1; junctional adhesion molecule 1; Junctional adhesion molecule A; KAT; Ly106; PAM-1; Platelet adhesion molecule 1; platelet F11 receptor; UNQ264/PRO301;
Gene ID (Entrez) 116479
Gene Symbol F11r
Interassay CV <12%
Intraassay CV <10%
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Shipping Condition Wet or Dry Ice
Storage Requirements Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Target JAM-A (CD321)
Test Time 1 hr 20 min
Total Assay Time 4 hr 45 min
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WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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