Invitrogen™ Rat MMP-2 ELISA Kit

Rat MMP-2 quantitates rat MMP-2 in serum, plasma, supernatant.

Manufacturer:  Invitrogen™ ERA42RBX5

Catalog No. ERA42RBX5

Description

Rat MMP-2 quantitates rat MMP-2 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant rat MMP-2.

MMP (matrix metalloproteinase) are proteolytic enzymes capable of degrading connective tissue components. MMP have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role in regulating angiogenesis. MMP2 is synthesized as a 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids, and contains the basic structure of propeptide, catalytic, and hemopexin domains. The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. Functionally, MMP2 is involved in tissue remodeling. Mutations in MMP-2 gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms of MMP-2 have been found.

Specifications

• For Use With (Equipment): Colorimetric Microplate Reader
• Conjugate: Biotin
• Label or Dye: HRP
• Gene ID (Entrez): P33436
• Regulatory Status: Research Use Only
• Storage Requirements: 2°C to 8°C
• Total Assay Time: 4 hr 45 min
• Interassay CV: <12%
• Quantity: 5 x 96 Tests
• Assay Sensitivity: 0.6 ng/mL

• Product Type: ELISA
• Accession Number: 72 kDa gelatinase; 72 kDa type IV collagenase; 72 kDa type IV collagenase isoform a preproprotein; 72 kD gelatinase; 72 kD type IV collagenase; 72 kDa gelatinase; 72 kDa type IV collagenase; CLG 4 A; CLG4; CLG4A; Collagenase; collagenase type IV-A; endopeptidase; GelA; gelatinase A; Gelatinase alpha; Mat; matrix metallo protease; matrix metallopeptidase 2; matrix metallopeptidase 2 (gelatinase A, 72 kDa gelatinase, 72 kDa type IV collagenase); matrix metalloprotease 2; matrix metalloproteinase; matrix metalloproteinase 2; matrix metalloproteinase 2 (72 KDa type IV collagenase); matrix metalloproteinase-2; matrix metalloproteinase-II; MMP; MMP2; MMP-2; MMP-2 protein; MMP-II; MMPs; MONA; neutrophil gelatinase; PEX; Progelatinase A; TBE1; TBE-1; wu:fa99h12; wu:fk89d01
• Assay Range: 0.614 to 150 ng/mL
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Sample Volume: Supernatant, 50 µL; Plasma, 50 µL; Serum, 50 µL
• Test Time: 1 hr 20 min
• Target: MMP2
• Intraassay CV: <10%
• Sample Type: Serum, Plasma, Supernatant
• Gene Symbol: MMP2