Invitrogen™ Rat NFKB-p105 ELISA Kit

Catalog No.	Quantity
EEL138	96 Tests

Catalog No. EEL138 Supplier Invitrogen™ Supplier No. EEL138

Description

ELISA

NF-kB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. It is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. NFKB1 (p50), a 50KDa functional sub-unit of NF-kB, is a member of Rel protein family. It is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NF-kB factor. NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-bidning. Pathological role of NF-kB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.

Specifications

• Accession Number: Q63369
• Assay Range: 0.16 to 10 ng/mL
• Assay Sensitivity: 0.1 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 81736
• Gene Symbol: NFKB1
• Interassay CV: <10%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: NFkB p105
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 30 mins