Invitrogen™ Rat Osteocalcin ELISA Kit

Catalog No.	Quantity
EEL123	96 Tests

Catalog No. EEL123 Supplier Invitrogen™ Supplier No. EEL123

Description

ELISA

The Rat Osteocalcin ELISA quantitates Osteocalcin in serum, plasma, and other biological fluids The Rat Osteocalcin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

Specifications

• Accession Number: P04640
• Assay Range: 0.78-50 ng/mL
• Assay Sensitivity: 0.47 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Osteocalcin/matrix Gla protein
• Sample Type: Plasma, Serum
• Target Species: Rat
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Bglap; Bglap1; Bglap2; BGP; Bgpr; Bgpra; bone gamma carboxyglutamate protein; bone gamma carboxyglutamate protein 1; bone gamma-carboxyglutamate (gla) protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate protein; bone gamma-carboxyglutamate protein 2; bone gamma-carboxyglutamate protein osteocalcin; bone gamma-carboxyglutamate; BGLAP; bone gamma-carboxyglutamic acid (Gla) protein (osteocalcin); bone Gla protein; bone gla protein BGP; bone Gla-protein; gamma-carbooxyglutamate (gla) protein; Gamma-carboxyglutamic acid-containing protein; GLA; Gla protein precusor; mOC-A; OC; OCN; OG1; Osteocalcin; PMF1; RP11-54H19.5; unnamed protein productBglap; Bglap1; Bglap2; BGP; Bgpr; Bgpra; bone gamma carboxyglutamate protein; bone gamma carboxyglutamate protein 1; bone gamma-carboxyglutamate (gla) protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate protein; bone gamma-carboxyglutamate protein 2; bone gamma-carboxyglutamate protein osteocalcin; bone gamma-carboxyglutamate; BGLAP; bone gamma-carboxyglutamic acid (Gla) protein (osteocalcin); bone Gla protein; bone gla protein BGP; bone Gla-protein; gamma-carbooxyglutamate (gla) protein; Gamma-carboxyglutamic acid-containing protein; GLA; Gla protein precusor; mOC-A; OC; OCN; OG1; Osteocalcin; PMF1; RP11-54H19.5; unnamed protein product;
• Gene ID (Entrez): 25295
• Gene Symbol: BGLAP
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Osteocalcin
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min