R&D Systems™ Recombinant Rat OX40 Ligand/TNFSF4 Protein

Catalog No.	Quantity
8640OX025	25 μg

Catalog No. 8640OX025 Supplier R&D Systems™ Supplier No. 8640OX025

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.05-0.25 μg/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050 ).

Specifications

• Accession Number: Q9Z2P3
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
• Gene ID (Entrez): 7292
• Molecular Weight (g/mol): M.W. (Observed): 17-31 kDa, reducing conditions; M.W. (theoretical): 18 kDa
• Quantity: 25 μg
• Source: Mouse myeloma cell line, NS0-derived rat OX40 Ligand/TNFSF4 protein Gln49-Leu199, with an N-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CD134 ligand, CD134L, CD252, CD252 antigen, Glycoprotein Gp34, gp34, OX40 antigen ligand, OX40 ligand, OX40L, OX-40L, OX4OL, TAX transcriptionally-activated glycoprotein 1, tax-transcriptionally activated glycoprotein 1 (34kD), tax-transcriptionally activated glycoprotein 1, 34kD, TNFSF4, tumor necrosis factor (ligand) superfamily, member 4, tumor necrosis factor ligand superfamily member 4, TXGP1
• Common Name: OX40 Ligand/TNFSF4
• Gene Symbol: Tnfsf4
• Biological Activity: Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is typically 0.05-0.25μg/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.
• Conjugate: Unconjugated
• Species: Rat
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 250 μg/mL in PBS.
• Expression System: Mouse myeloma cell line, NS0-derived Gln49-Leu199, with an N-terminal 6-His tag
• Purity or Quality Grade: >95%, by SDS-PAGE with silver staining.
• Protein: OX40 Ligand/TNFSF4