Gibco™ Rat Primary Cortical Astrocytes

Catalog No.	Quantity
N7745100	1 x 10^6 cells

Catalog No. N7745100 Supplier Gibco™ Supplier No. N7745100

Description

Gibco Rat Primary Cortical Astrocytes provide neuroscience researchers with a ready-to-use, highly pure source of astrocytes for experiments.

Gibco Rat Primary Cortical Astrocytes are isolated and cryopreserved from the cortex of Sprague Dawley rats at day E19 of gestation. The astrocytes are passaged only once before cryopreservation and consistently yield post-thaw viability of 70% or greater. Each vial contains 1 million cells per mL and provides a flexible, ready-to-use, and quality alternative to fresh astrocytes.

Rat Primary Cortical Astrocyte details

• Exhibit ≥70% viability upon thawing
• Stain >80% positive for the astrocyte specific marker glial fibrillary acid protein (GFAP)
• Stain ≤10% positive for neuron and oligodendrocyte specific markers galactocerebroside (GalC) and doublecortin (DCX) or tubulin beta-3 (TUBB3)
• Exhibit a doubling time of approximately nine days at P2
• Can be expanded in culture for at least one passage

Specifications

• Content And Storage: Store in liquid nitrogen, vapor-phase.
• Age: Fetal
• Cell Type: Glial Cells
• Culture Type: Mammalian Cell Culture
• Form: Cryopreserved
• Species: Rat
• Tissue: Cortex
• No. of Cells: 1 x 10⁶ cells
• Product Type: Rat Cortical Astrocyte
• Quantity: 1 x 10⁶ cells
• Tested For: Cell Purity, Sterility, Viability, Mycoplasma

Frequently Asked Questions (FAQs)

What are some of the key applications for astrocytes?

Astrocytes can be used to research cell transplantation, intracellular transport, ion-channel function, toxicity, receptor signaling, analyses of electrophysiological properties, neurotransmitter release, and evaluation of interaction between neurons and astrocytes.

I used rat primary cortical astrocytes from another company and observed cell aggregates after thawing but not with the astrocytes from your company. Why?

Gibco Rat Primary Cortical Astrocytes were dissociated into single cells before cryopreservation, which gives an advantage to control the number of cells to plate.

How can I coat Gibco Rat Primary Cortical Astrocytes on glass coverslips?

We recommend cleaning the coverslips and then coating them with 0.01% poly-D-lysine in double distilled water.

What is the doubling time of Gibco Rat Primary Cortical Astrocytes?

The doubling time of Gibco Rat Primary Cortical Astrocytes is about 8.7 days.

For how many passages can I expand Gibco Rat Primary Cortical Astrocytes?

Gibco Rat Primary Cortical Astrocytes can be thawed, recovered, and proliferated up to three passages to yield 1.5-2 fold increase of cells.

What is the % purity of Gibco Rat Primary Cortical Astrocytes?

Cell purity was evaluated in terms of expression of the astrocyte-specific marker, GFAP. More than 80% of the cells were positive for GFAP marker, and the expression of the neuronal marker (DCX) and oligodendrocyte marker (GalC) was less than 10%.

What is the viability of Gibco Rat Primary Cortical Astrocytes once thawed?

Upon thawing, the vial has more than 70% viability, which will give more than 1 million live cells.

Is there any difference between primary rat cortical astrocytes isolated from fetal, postnatal, and adult tissue?

Primary rat astrocytes are typically isolated from late fetal or early postnatal brain because the resulting astrocytes are more viable in culture and of higher purity than astrocytes from adult brain.

What is the difference, if any, between cortical and hippocampal astrocytes?

There are few known differences between cortical and hippocampal astrocytes. However, it has been reported that astrocytes from different regions of the brain show a differential sensitivity to ischemic injury.
- Zhao, Gang and Flavin, Michael, Differential sensitivity of rat hippocampal and cortical astrocytes to oxygen-glucose deprivation injury. Neuroscience Letters, 2000; 285: 177-180.
- Xu C. et al., Feeder-free growth of undifferentiated human embryonic stem cells. Nat Biotechnol, 2001; 19(10): 971-974.

At what passage were Gibco Rat Primary Cortical Astrocytes cryopreserved?

Gibco Rat Primary Cortical Astrocytes were cryopreserved in growth medium containing 10% DMSO at passage 1 (P1).

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.