Invitrogen™ Rat TIMP-2 ELISA Kit

Description

ELISA

Rat TIMP-2 quantitates rat TIMP-2 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant rat TIMP-2.

TIMP-1 and TIMP-2 are inhibitory enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Specifications

• Accession Number: collagenase inhibitor; CSC-21 K; D11Bwg1104e; DDC8; Metalloproteinase inhibitor 2; metalloproteinase inhibitor precursor; metalloproteinase inhibitor TIMP-2; similar to metalloproteinase inhibitor; testicular secretory protein Li 57; TIMP 2; TIMP metallopeptidase inhibitor 2; Timp2; TIMP-2; TIMP-2 protein; tissue inhibitor of matrix metalloproteinase-2; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinase-2; tissue inhibitor of metalloproteinases 2; tissue inhibitor of mettaloproteinase 2
• Assay Range: 20 to 5,000 pg/mL
• Assay Sensitivity: 20 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 29543
• Gene Symbol: TIMP2
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: TIMP2
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins