Gibco™ Rat VEGF-165 Recombinant Protein, PeproTech®

Catalog No.	Quantity
400-31-50UG	50 μg
40031100UG	100 μg
400-31-10UG	10 μg
400-31-1MG	1 mg
40031250UG	250 μg
400-31-2UG	2 μg
40031500UG	500 μg

Catalog No. 400-31-50UG Supplier Gibco™ Supplier No. 4003150UG

Description

Recombinant Protein

Recombinant Rat VEGF 165 is a 38.5 kDa, disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.

Specifications

• Accession Number: P16612
• For Use With (Application): Control, Western Blot Control, ELISA Standard, Bioactivity
• Formulation: Protein with no preservative
• Gene ID (Entrez): 83785
• Molecular Weight (g/mol): 38.5 kDa
• Name: Rat VEGF-165
• Purification Method: Purified
• Quantity: 50 μg
• Source: E. coli
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/μg
• Gene Alias: H-VEGF-165; MVCD1; M-VEGF-165; vascular endothelial growth factor 165b; vascular endothelial growth factor A; vascular endothelial growth factor A121; vascular endothelial growth factor A165; vascular permeability factor; VEGF; VEGF164; VEGF165; Vegfa; VEGF-A; VPF
• Common Name: VEGF-165
• Gene Symbol: Vegfa
• Biological Activity: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC). The expected ED50 is 4.0-8.0 ng/ml
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
• Content And Storage: -20°C
• Activity: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC). The expected ED50 is 4.0-8.0 ng/ml.
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.