Invitrogen™ VEGFC Rat ELISA Kit

For the quantitative detection of rat VEGF-C.

Supplier:  Invitrogen™ BMS6262TEN

Catalog No. 50-183-50

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to rat VEGF-C
Biotin-Conjugate anti-rat VEGF-C monoclonal antibody
Streptavidin-HRP
Rat VEGF-C Standard lyophilized, 6ng/mL upon reconstitution
Sample Diluent
Conjugate Diluent
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

The Rat Vascular Endothelial Growth Factor C (Rt VEGFC) ELISA quantitates Rt VEGFC in rat serum or cell culture medium. The assay will exclusively recognize both natural and recombinant rat VEGFC. Principle of the method The Rat VEGFC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D.

Specifications

• Accession Number: O35757
• Assay Sensitivity: 27.0 pg/mL
• Product Type: ELISA Kit
• Target Species: Rat
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Vegfc
• Intraassay CV: 5.1%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Rat
• Total Assay Time: 3 hr. 30 min.

• Assay Range: 47.0-3,000 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 114111
• Interassay CV: 2.7%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 10 x 96 Tests
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
• Synonym: VRP, FLT-4 ligand
• Test Time: 1 hr. 20 min.

For Research Use Only