BD Horizon™ RB670 Streptavidin

Description

Streptavidin is a non-glycosylated protein that is prepared chromatographically from the bacterium Streptomyces avidinii.

Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with fluorochromes, streptavidin has been widely used with biotin-conjugated antibodies and other biotinylated specific-binding molecules (eg, recombinant proteins and lectins) to stain cells and tissues for subsequent multiparameter analysis by flow cytometry, fluorescence microscopy and imaging. Likewise, when conjugated with an enzyme (eg, Horseradish Peroxidase or Alkaline Phosphatase) and coupled with a colorimetric or luminescent substrate development system, streptavidin has found widespread use along with biotinylated antibodies in a number of applications including Western blot, ELISA, ELISPOT, immunocytochemistry and immunohistochemistry.

Specifications

• Concentration: 0.1 mg/mL
• Conjugate: RB670
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Quantity: 100 μg
• Regulatory Status: RUO
• Content And Storage: Streptavidin was conjugated with dye under optimum conditions, and unconjugated Streptavidin and free dye were removed Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Form: Liquid
• For Use With (Application): Flow Cytometry