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Santa Cruz Biotechnology RBC LYSIS BUFFER 10X 100ML
Supplier: Santa Cruz Biotechnology SC296258
Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use. RBC lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples. The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use. NOTE: The blood sample must be <1 month old and been stored in anticoagulants (i.e. EDTA, heparin, and citrate).
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